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Publication type: Baccalaureate Thesis

Year: 2014

Author(s): Janjic, Klara

Title: Reproduktionsbiotechnologische Studien bei STRA-Wildfangmäusen.

Other title: Studies of reproductive biotechnology on wild-derived mouse strain STRA

Source: Bakkalaureatsarbeit, Vet. Med. Univ. Wien, pp. 40.


Kolbe Thomas

Müller Mathias

Vetmed Research Units:
Institute of Laboratory Animal Science

Graduation date: 01.07.14

Current methods for superovulation as one of the assisted reproductive technologies (ARTs) only obtained poor results on the wild-derived strain STRA. The aim of this study was to verify this evidence and, in case of proving true, to develop an effective method for superovulation of STRA females. First, it was analysed if vaginal plugs are a reliable sign for occurred mating of STRA mice. One aspect was to examine strain specific anatomical differences between males of STRA and laboratory mouse strains. For this purpose Gll. vesiculares and coagulating glands of juvenile and adult STRA males in single or group housing were examined for macroscopic irregularities as well as for their weight in proportion to age, housing condition and body weight. Comparable groups of males of a laboratory mouse strain (C57BL/6) were used as control. While the control group showed a clear correlation between gland and body weight, for STRA mice no significant correlations could be found. Furthermore tests were conducted to find out if STRA males are able to produce vaginal plugs in STRA females. For that STRA males and females were mated and checked for vaginal plugs in the mornings. For testing of probably missed out plugs and resulting gestation, uteri of the females were removed and stained to detect implantation sites. In addition to that STRA males and females were mated respectively with the opposite sex of a laboratory mouse strain (CD1). Females were again checked for vaginal plugs and gestation. Only one STRA female was observed to have a vaginal plug. Since STRA mice show increased physical activity and less tame behaviour than mice of laboratory strains, it was assumed that increased levels of stress are responsible for limited reproduction. Thus an enzyme immunoassay was conducted to measure the concentration of glucocorticoid metabolites in faecal samples and to estimate extents of stress levels. Same samples were used to measure concentrations oestrogen and draw conclusions to the length of the oestrous cycle of STRA females. Results confirmed that stress levels are increased in STRA mice. Patterns of measured oestrogen concentrations could not be interpreted, in order of that it was not possible to determine clear lengths of oestrus cycles. For the development of a successful superovulation regime, two groups of STRA females were formed with different age (“old” = from 8 weeks on and “young” = 4-7 weeks), using 3 different hormone doses on them (PMSG an hCG each 2 IU, 10 IU or 20 IU). The aim was to reach a high number of embryos for cryopreservation. The “old” group achieved best results using a hormone dose of 2 IU, while a dose of 10 IU was the most successful for mice of the “young” group. This study demonstrates that reproduction in the wild-derived strain STRA clearly differs from laboratory mouse strains. However successful superovulation of STRA mice can be achieved by using adapted regimes.

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