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Selected Publication:

Publication type: Diploma Thesis

Year: 2015

Author(s): Neels, Tania

Title: Combi-vet (r) Narkosekammer versus Restrainer zur intravenösen Applikation bei Mäusen.

Other title: Combi-vet (r) Inductionchamber versus Restrainer for the intravenous route of administration in mice

Source: Diplomarbeit, Vet. Med. Univ. Wien, pp. 44.


Advisor(s):

Rülicke Thomas

Reviewer(s):
Russold Elena

Vetmed Research Units:
Institute of Laboratory Animal Science


Abstract:
Intravenous injection into the lateral tail vein of mice is a commonly used route of administration in experimental studies. A special induction chamber was developed to refine this procedure especially for use by untrained personnel. In this new technique when the mouse is under anaesthesia one must lead the tail through a small hole padded with foam, located in the lid of the chamber. This procedure should create pressure on the blood vessels, which should eliminate the need for tail warming to dilate veins for a better visibility. Thereby injection by this method is supposed to be easier and faster regardless of practical experience. The aim of the study was to assess the applicability of the induction chamber in contrast to the commonly used restrainer by using several objective and subjective criteria. In order to evaluate the stress response of mice to the two intravenous administration methods we used non-invasive parameters such as nest complexity scoring, body weight and faecal corticosterone metabolite levels. As test animals we used females and males of an albino and a coloured mouse strain. The values of nest scoring and body weight showed no significant differences between treatment groups and the control. However, faecal corticosterone metabolites showed an increase for all groups after the treatment but not the control group. The increase was not significant. The males of groups 1B (Restrainer with i.v. injection), 2A (induction chamber without i.v. injection) and 2B (induction chamber with i.v. injection) showed a significant higher excretion of faecal corticosterone metabolites from day -2 to day 0 (day of treatment) in comparison to the control group. Our assessment of applicability (such as hit ratio of the tail vein or length of procedures) tested by untrained personnel resulted in a preference for the restrainer over the induction chamber.


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