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Selected Publication:

Type of publication: Doctoral Thesis
Type of document:

Year: 2010

Authors: Reiter, EV

Title: Development and evaluation of clean up methods in Ochratoxin A determination.

Other title: Entwicklung und Evaluierung von Probenvorbereitungsmethoden für die Bestimmung von Ochratoxin A

Source: Dissertation, Vet. Med. Univ. Wien, pp. 170.


Advisor(s):

Razzazi-Fazeli Ebrahim

Reviewer(s):
Cichna-Markl, Margit
Razzazi-Fazeli Ebrahim

Vetmed Research Units:
Institute of Animal Nutrition and Functional Plant Compounds


Graduation date: 07.10.10


Abstract:
Two sample preparation techniques based on the formation of antigen-antibody-complexes (Ag-Ab-complexes), for the clean up of ochratoxin A (OTA) contaminated samples, were developed. While in the first technology, the immuno-ultrafiltration (IUF), the antibodies were used in free form, in the second technique, the sol-gel technologie antibodies were entrapped in the pores of a glass. The IUF is a rapid and easy to use sample pre-treatment method, which can be considered as an alternative to most commonly used immunoaffinity clean up, since most common immobilisation procedures are labour and time intensive. In the second method sol-gel immunoaffinity columns selective for OTA were prepared and optimized for the clean up of food and feed samples. Advantages of the sol-gel technology are the ease of production of the columns, followed by a well known clean up procedure. The reusability is guaranteed by reconditioning the sol-gel columns with PBS. Both newly developed techniques were successfully applied in the analyses of cereal crops, where satisfactory recoveries were obtained.

Keywords:
Ochratoxin A / sample preparation / immuno-ultrafiltration / sol-gel immunoaffinity chromatography / cereals


Publication(s) resulting from University thesis:

Reiter, EV; Vouk, F; Böhm, J; Razzazi-Fazeli, E (2010): Aflatoxins in rice - A limited survey of products marketed in Austria. Food Control (21), 7 988-991.

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