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Type of publication: Master Thesis
Type of document:

Year: 2009

Authors: Schimke, Magdalena

Title: The effect of simulated diabetes mellitus on adult rat cardiac fibroblasts (ARCF).

Source: Master Thesis, Vet. Med. Univ. Wien, pp. 75.


Klein Dieter

Waldhäusl, Werner

Vetmed Research Units:

Diabetes Mellitus (DM) is the fastest emerging disease in industrialized countries. This metabolic disorder is caused by the inability of the pancreas to produce the hormone insulin, which is necessary to metabolize glucose into energy, or the body’s cells inability to respond to it. The consequences of DM are multiple and can lead over time to the induction of a diabetic cardiomyopathy, which adversely affects cardiac structure/function. A major consequence is the induction of cardiac fibrosis. In this project, adult cardiac rat fibroblasts (ARCF) grown under controlled conditions (5mM glucose) or simulated diabetic conditions (25mM glucose) are used to investigate mechanisms that may lead to DM induced cardiac fibrosis. Of particular interest is the enzymatic attachment of glucose to signaling proteins of the TGF-beta and/or Raf/Ras MAPK pathway, which may be crucial in protein and/or collagen synthesis. We hypothesize that high glucose may interfere with the normal signaling of the extracellular regulated kinase (ERK) via enzymatic glycosylation (O-linked GlcNAcylation) of key regulatory phosphorylation sites, leading to increases in protein and/or collagen synthesis.

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