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Type of publication: Diploma Thesis
Type of document:

Year: 2011

Authors: Kreilmeier, Theresa

Title: Immunhistochemical characterization of the retina in the vitamin D receptor-deficient VDRΔ/Δ mouse.

Other title: Immunhistochemische Untersuchungen an der Netzhaut der Vitamin D Rezeptor-defizienten VDRΔ/Δ-Maus

Source: Diplomarbeit, Vet. Med. Univ. Wien, pp. 48.


Authors Vetmeduni Vienna:

Kreilmeier-Berger Theresa

Advisor(s):
Glösmann Martin

Reviewer(s):
Egerbacher Monika

Vetmed Research Units:
Institute of Physiology, Pathohysiology and Biophysics, Unit of Physiology, Pathophysiology, and Experimental Endocrinology


Graduation date: 22.06.11


Abstract:
Vitamin D receptor (VDR) expression has been detected in many mammalian tissues. Abundant evidence suggests a role of vitamin D in ocular function and dysfunction, including immunological responses, glaucoma, myopia, and age-related macular degeneration. It is still unclear, however, whether a VDR is expressed in the mammalian retina in vivo,In this study, I sought to identify sites of VDR expression in the adult mouse retina and to compare the structure of the retina and its major cell types between wild type and VDRΔ/Δ mutant mice to determine the effects of the lack of a functional VDR. X-gal (β-galactosidase) histochemistry and lacZ immunohistochemistry were used to detect lacZ reporter gene expression in the retina of adult VDRΔ/Δ–lacZ mice supplemented with calcium, phosphorus, and lactose. DAPI staining and immunohistochemistry with retinal cell type-specific markers were performed on frozen sections of wild type and VDRΔ/Δ–lacZ mouse retinae. In the adult mouse retina, VDR is expressed in the proximal inner nuclear layer and in the ganglion cell layer. The VDRΔ/Δ mutation does not affect retinal layering and the structure of the major retinal cell types. In the retina of VDRΔ/Δ mutant mice, parvalbumin expression in a subset of amacrine and ganglion cells is significantly lower compared to wild type controls. This is similar to findings in the kidney of VDR null mice. In one week old mice, the VDRΔ/Δ mutation appears to be associated with increased apoptosis. Together, the data suggest a role of vitamin D signaling in amacrine and ganglion cell function.


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