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Selected Publication:

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Type of publication: Master Thesis
Type of document:

Year: 2011

Authors: Curkić, Ismeta

Title: Generation of recombinant antibodies against HIV clade C envelope.

Other title: Generierung von rekombinanten Antikörpern gegen die HIV Stamm C Hülle

Source: Master Thesis, Vet. Med. Univ. Wien, pp. 91.


Advisor(s):

Ruprecht Ruth M.
Klein Dieter

Reviewer(s):
Saalmüller Armin

Vetmed Research Units:
VetCore


Graduation date: 22.09.11


Abstract:
The human immunodeficiency virus (HIV) is the causative agent of the acquired immunodeficiency syndrome (AIDS) and is responsible for a worldwide epidemic with approximately 34 million infected individuals in 2009. The high prevalence of HIV-1 underlines the urgent need of a safe and effective vaccine. Generation of a broadly neutralizing monoclonal antibody (MAb) would not only reveal conserved epitopes within the viral envelope but also importantly, bring us a big step closer to finding an effective AIDS-vaccine. In the course of a vaccine study, two rhesus macaques (Macaca mulatta) were challenged with SHIV-1157ipEL-p after several immunizations. Interestingly, one of these monkeys remained aviremic, despite several challenges. Plasma from this monkey was used in another study to isolate mimotopes (sequences mimicking epitopes within the viral Env), located within the C-terminal tail of the viral gp41. In this study, these mimotopes were used to isolate antigen-specific B cells from rhesus monkeys. Single B cells were used to amplify variable regions of rhesus monkey immunoglobulin genes to express and characterize binding capabilities of mimotope-specific MAbs. We were able to isolate B cells from both rhesus macaques and amplified variable immunoglobulin genes. We successfully generated three antigen-specific MAbs, with one of these found to be specific to GFP.


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