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Type of publication: Baccalaureate Thesis
Type of document:

Year: 2012

Authors: Philippi Novak, Tabea

Title: Analyse des zellulären Transkriptoms von Felines Immundefizienz Virus-infizierten Zellen mithilfe von Next Generation Sequencing-Technologie.

Other title: Analysis of the cellular transcriptome in FIV-infected cells with Next-Gneration Sequencing technology

Source: Bakkalaureatsarbeit, Vet. Med. Univ. Wien, pp. 57.


Advisor(s):

Klein Dieter

Reviewer(s):
Walter Ingrid

Vetmed Research Units:
VetCore


Graduation date: 05.10.12


Abstract:
Feline immunodeficiency virus (FIV) is a lentivirus causing an AIDS-like disease in cats. Animals infected by FIV show similar characteristics to human infections with human immunodeficiency virus (HIV), e.g. reductions in the ratios of CD4+ to CD8+ T-cells. The differences in pathogenicity of FIV strains can mirror the range of symptomes associated with HIV infection. The aim of this study is to investigate effectors involved in transcription of cellular genes in view of discover similar affected pathways to HIV-infection. Therefore we used two different FIV virus strains, FIV Petaluma and FIV Glasgow 8, which are known to display different pathogenicities. FIV Petaluma (PET) is considered to be a strain with low virulence which causes only low plasma viral loads in infected cats and has no effect on the numbers of CD4+ and CD8+ T-cells whereas highly pathogenic FIV Glasgow 8 strain (GL8) leads to high viral loads and significant reductions of CD4+/CD8+ T-cell ratios. The infectiontarget used was CrandellÂ’s feline kidney cells (CrFK).


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