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Type of publication: Master Thesis
Type of document:

Year: 2013

Authors: Schreiner, Melanie

Title: Expression of the VD1/RPD2 α1-neuropeptide during development of a basal gastropod.

Other title: Expression des VD1/RPD2 alpha 1-Neuropeptids während der Entwicklung eines basalen Gastropoden

Source: Master Thesis, Vet. Med. Univ. Wien, pp. 27.


Glösmann Martin

Haszprunar Gerhard

Vetmed Research Units:

Graduation date: 09.01.13

Several molluscs express the VD1/RPD2 α1-neuropeptide in their central nervous system (CNS). It was first detected in the pulmonate gastropod Lymnaea stagnalis, where it is predominantly expressed in the visceral and right parietal ganglion. Further examinations revealed the expression of that neuroactive compound in the CNS of other adult gastropods, bivalves and cephalopods. The present study examines the expression of the VD1/RPD2 α1-neuropeptide during development of the patellogastropod Lottia kogamogai, which is considered as a basal gastropod. With the use of immunocytochemistry and confocal laser scanning microscopy (CLSM) the distribution of the VD1/RPD2 α1-neuropeptide was examined during larval development and in adults of L. kogamogai. Trochophore and pretorsional veliger larvae were devoid of signal. The VD1/RPD2 α1 neuropeptide first appears in the posttorsional veliger. The nervous system of veliger larvae prior to metamorphosis shows prominent tentacle nerves, anlagen for the future cerebral ganglia, a visceral loop and anlagen for the pedal ganglia with foot nerves branching into the anterior and posterior side of the foot. VD1/RPD2 α1-neuropeptide expression is restricted to the anlage of the future cerebral ganglia, the visceral loop and the anlage of the pedal ganglia. In the adult animal, the cerebral ganglia have a high expression of that neuroactive compound. The results obtained herein show that the VD1/RPD2 α1-neuropeptide is not present in larval neural components that are lost during metamorphosis, such as the apical organ. By contrast, the expression is restricted to the ganglionic anlagen of the future adult CNS. This strongly suggests that the VD1/RPD2 α1-neuropeptide is primarily important for postmetamorphic life cycle stages of L. kogamogai. Whether this holds true for other gastropods (and molluscs) as well needs to be assessed by further comparative neurodevelopmental studies.

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