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Publication type: Master Thesis

Year: 2010

Author(s): Pölzler, Thomas

Title: Praktische Evaluierung eines Schnelltests zur Diagnose von Campylobacter spp. in Geflügelkot und Verifizierung mittels quantitativer Kultur sowie Real-Time PCR.

Source: Master Thesis, Vet. Med. Univ. Wien, pp. 75.


Advisor(s):

Hess Michael
Köfer Josef

Vetmed Research Units:
University Clinic for Poultry and Fish Medicine


Graduation date: 01.03.10


Abstract:
In the past thirty years human campylobacteriosis has emerged to be the most common cause of acute gastroenteritis in the industrialised countries. Efforts have been made to decrease the incidence of Campylobacter infections. A very important source of infection is poultry meat. The results from the Dutch CARMA project strongly indicate that meat from broiler flocks shedding Campylobacter in higher concentrations then 7 log10 cfu/g faeces involve greater risk for consumers to suffer from Campylobacteriosis. If those herds could be identified and separated from fresh meat production most products yielding high probabilities of illness could be removed from the food chain (NAUTA and HAVELAAR, 2008). To identify high shedder broiler flocks an easy to use rapid test has been developed (WADL, 2008). The rapid test was verified within the context of the Commissions Decision 2007/516/EC called “Base-line-study Campylobacter broiler”. Results stood within the detection limit of ≥ 6.7 log10 cfu/g faeces for C. jejuni and ≥ 7.1 log10 cfu/g for C. coli under laboratory conditions (WADL, 2008). Out of 187 examined samples 102 have been tested positive and 69 negative by the rapid test. All results were confirmed by the reference method ISO 10272. The test showed only 5 false positive or negative results. Applied on the results of the reference method, the rapid test showed a sensitivity of 89.5 and a specificity of 94.5 %. For the reference method the prevalence was 61 % in the sample period. Additionally all samples were examined by real-time PCR. With this method the results of the rapid test and the reference method could be confirmed and a prevalence of 71.7 % was calculated for the sample period.

Keywords:
Campylobacter, rapid test, broiler, chicken, real time PCR


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