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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2006

Authors: Wolfesberger, B; Walter, I; Hoelzl, C; Thalhammer, JG; Egerbacher, M

Title: Antineoplastic effect of the cyclooxygenase inhibitor meloxicam on canine osteosarcoma cells.

Source: Res Vet Sci. 2006; 80(3):308-316



Authors Vetmeduni Vienna:

Egerbacher Monika
Thalhammer Johann
Walter Ingrid
Wolfesberger Birgitt

Vetmed Research Units
University Clinic for Small Animals, Clinical Unit of Internal Medicine Small Animals
Institute of Topographical Anatomy


Abstract:
A decisive role in cancer development has been attributed to cyclooxygenase-2 (COX-2) activity, but the significance of COX-2 inhibitors in cancer treatment still needs to be thoroughly investigated. We studied the influence of meloxicam, a non-steroidal antiinflammatory drug with preferential inhibitory effects on COX-2 compared to COX-1, on canine osteosarcoma (D-17) cells. We demonstrated that D-17 cells expressed mRNA and COX-2 protein. Treatment with meloxicam induced a time- and dose-dependent inhibition of cellular growth. To determine if apoptosis plays a role in meloxicam-induced cell death, we performed agarose gel electrophoresis and found a DNA-ladder pattern, typically seen in apoptosis, as well as early apoptotic changes by Annexin V tests. Furthermore, electron microscopy revealed ultrastructural alterations typical of apoptosis. Quantification of apoptotic cells by immunohistochemical staining of caspase 3 confirmed the results. However, further studies with meloxicam are necessary to assess its potential use for treatment of osteosarcomas in dogs.

Keywords Pubmed: Animals
Antineoplastic Agents/pharmacology*
Apoptosis/drug effects
Blotting, Western/veterinary
Bone Neoplasms/drug therapy*
Bone Neoplasms/pathology
Bone Neoplasms/veterinary*
Caspase 3
Caspases/metabolism
Cell Line, Tumor
Cell Proliferation/drug effects
Cyclooxygenase 2/genetics
Cyclooxygenase 2/metabolism
Cyclooxygenase 2 Inhibitors/pharmacology*
Dog Diseases/drug therapy*
Dog Diseases/pathology
Dogs
Immunohistochemistry/veterinary
Microscopy, Electron, Transmission/veterinary
Microscopy, Fluorescence/veterinary
Osteosarcoma/drug therapy*
Osteosarcoma/pathology
Osteosarcoma/veterinary*
RNA, Neoplasm/chemistry
RNA, Neoplasm/genetics
Reverse Transcriptase Polymerase Chain Reaction/veterinary
Thiazines/pharmacology*
Thiazoles/pharmacology*


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