Introduction Thermophilic Campylobacter (C.), mainly C. jejuni and C. coli are recognized as the most common cause of acute bacterial enteritis in humans worldwide. Contaminated animal products, especially poultry meat, are considered as major vehicles of infection since Campylobacter can be present at high numbers in these animals without clinical signs. In addition to this high prevalence, increasing resistance to antimicrobial drugs has been documented in Campylobacter isolates obtained from both poultry and humans. This is of particular concern because quinolones, macrolides and tetracyclines are used in humans to treat campylobacteriosis. Material and methods In the present study the antibiotic resistance of 183 Campylobacter isolates (134 isolates from poultry, 49 isolates from swine and cattle) was investigated, using microdilution and the breakpoint-method. In total, 125 C. jejuni and 58 C. coli were investigated. Results Multiresistance was observed in 38.8 % of poultry and 30.6 % of swine/cattle isolates. Nearly 50 % of the poultry isolates were resistant to quinolones, in contrast to about one third of isolates obtained from swine and cattle. Resistance to erythromycine, tylosine and tetracycline of poultry isolates was found to be 14.2, 61.9 and 33.6 %, whereas values of 36.7, 89.9 and 53.1 % were obtained for the swine and cattle isolates, respectively. Except for spectinomycine and penicilline G against which 53.0 and 74.6 % of the poultry isolates and 61.2 and 69.4 % of the swine/cattle isolates showed resistance, only a limited number of bacteria obtained from poultry were resistant against aminoglycosides and penicillines. Furthermore, 57.1 % of the swine and cattle isolates were resistant to streptomycine. Conclusion Our results demonstrate the necessity of a continous surveillance as given by the EU-directive 99/2003/EG.