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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2006

Author(s): Gerner, W; Denyer, MS; Takamatsu, HH; Wileman, TE; Wiesmüller, KH; Pfaff, E; Saalmüller, A

Title: Identification of novel foot-and-mouth disease virus specific T-cell epitopes in c/c and d/d haplotype miniature swine.

Source: Virus Res. 2006; 121(2):223-228



Authors Vetmeduni Vienna:

Gerner Wilhelm
Saalmüller Armin

Vetmed Research Units
Institute of Immunology


Abstract:
To identify foot-and-mouth disease virus (FMDV) specific T-cell epitopes within the non-structural protein 3D in swine, pentadecapeptides were tested in proliferation and Interferon-gamma ELISPOT assays using lymphocytes from two strains of inbred miniature pigs (c/c and d/d haplotype) experimentally infected with FMDV. Lymphocytes of c/c pigs recognized peptides from three different regions in 3D, d/d lymphocytes recognized peptides from two regions, one of them being adjacent to an epitope of c/c pigs and comprising amino acid residues 346-370. Analyses of the response of d/d lymphocytes against peptides representing the structural protein 1A revealed another novel T-cell epitope. Investigation of the phenotype of responding lymphocytes showed a response of CD4(+)CD8(+)MHC-class-II(+) cells, identifying them as activated T-helper cells. This is the first report on FMDV specific T-cell epitopes recognized by swine leukocyte antigen (SLA) inbred swine and provides information useful for the design of novel vaccines against FMDV.

Keywords Pubmed: Amino Acid Sequence
Animals
Animals, Inbred Strains
Cells, Cultured
Enzyme-Linked Immunosorbent Assay
Epitopes, T-Lymphocyte/immunology*
Foot-and-Mouth Disease/immunology*
Foot-and-Mouth Disease Virus/immunology*
Haplotypes
Histocompatibility Antigens/immunology
Immunologic Memory
Interferon-gamma/analysis
Leukocytes, Mononuclear/immunology
Lymphocyte Activation
Molecular Sequence Data
Peptides/genetics
Peptides/immunology
Swine
Swine, Miniature
T-Cell Antigen Receptor Specificity
T-Lymphocytes/immunology*
T-Lymphocytes, Helper-Inducer/immunology
Viral Nonstructural Proteins/chemical synthesis
Viral Nonstructural Proteins/immunology


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