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Publication type: Journal Article
Document type: Full Paper

Year: 2007

Author(s): Gerner, W; Carr, BV; Wiesmüller, KH; Pfaff, E; Saalmüller, A; Charleston, B

Title: Identification of a novel foot-and-mouth disease virus specific T-cell epitope with immunodominant characteristics in cattle with MHC serotype A31.

Source: Vet Res. 2007; 38(4):565-572



Authors Vetmeduni Vienna:

Gerner Wilhelm
Saalmüller Armin

Vetmed Research Units
Institute of Immunology


Project(s): Entwicklung einer synthetischen Vakzine gegen die Maul-und-Klauenseuche


Abstract:
To identify foot-and-mouth disease virus (FMDV) specific T-cell epitopes within the entire polyprotein sequence of the virus, 442 overlapping pentadecapeptides were tested in proliferation assays using lymphocytes from cattle experimentally infected with FMDV. Four months post-infection cells from all investigated animals (n = 4) responded by proliferation and interferon-gamma production to a peptide located on the structural protein 1D (VP1), amino acid residues 66-80. Major histocompatibility complex (MHC) serotyping of the investigated cattle indicated that all animals shared the MHC serotype A31 which comprises the class II allele DRB3 0701. This may explain the common recognition of this newly discovered epitope. Responses to other peptides could only be observed in one animal and rapidly declined during the time course of the study. These observations point to an immunodominant role of this epitope located on the protein 1D in cattle with MHC serotype A31.

Keywords Pubmed: Animals
Cattle
Cattle Diseases/immunology*
Cattle Diseases/virology
Epitopes, T-Lymphocyte/immunology*
Foot-and-Mouth Disease/immunology*
Foot-and-Mouth Disease/virology*
Foot-and-Mouth Disease Virus/immunology*
Gene Expression Regulation
Histocompatibility Antigens Class II/classification
Histocompatibility Antigens Class II/immunology*
Immunodominant Epitopes/immunology*
Interferon-gamma/metabolism
Leukocytes, Mononuclear/metabolism
Male


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