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Type of publication: Journal Article
Type of document: Full Paper

Year: 2007

Authors: Wolfesberger, B; Guija de Arespacohaga, A; Willmann, M; Gerner, W; Miller, I; Schwendenwein, I; Kleiter, M; Egerbacher, M; Thalhammer, JG; Muellauer, L; Skalicky, M; Walter, I

Title: Expression of vascular endothelial growth factor and its receptors in canine lymphoma.

Source: J Comp Pathol. 2007; 137(1):30-40

Authors Vetmeduni Vienna:

Egerbacher Monika
Gerner Wilhelm
Guija-De-Arespacochaga Abigail
Kleiter Miriam
Miller Ingrid
Schwendenwein Ilse
Skalicky Monika
Thalhammer Johann
Walter Ingrid
Willmann Michael
Wolfesberger Birgitt

Vetmed Research Units
Clinical Pathology Platform
University Clinic for Small Animals, Clinical Unit of Internal Medicine Small Animals
Institute for Medical Biochemistry
Institute of Immunology
Institute of Morphology
Institute of Physiology, Pathohysiology and Biophysics, Unit of Physiology, Pathophysiology, and Experimental Endocrinology

Vascular endothelial growth factor (VEGF) stimulates endothelial cell proliferation and has a pivotal role in tumour angiogenesis. The expression of VEGF and its receptors VEGFR-1 and VEGFR-2 was examined immunohistochemically in 43 specimens of canine lymphoma and in six normal lymph nodes. Western blotting and reverse transcriptase polymerase chain reaction (RT-PCR) were performed to detect VEGF protein and mRNA, respectively. VEGF protein was expressed by 60% of the tumours with diffuse cytoplasmic labelling of the neoplastic cells. Endothelial cells, macrophages and plasma cells were also immunolabelled. VEGFR-1 was expressed by variable numbers of neoplastic cells in 54% of lymphoma specimens. VEGFR-1 was also expressed by macrophages, plasma cells, reticulum cells, and vascular endothelial cells. Macrophages and lymphocytes in germinal centres of normal lymph nodes were also immunoreactive with anti-VEGF and VEGFR-1. Most tumours did not express VEGFR-2 but in 7% of sections there was focal labelling of neoplastic and endothelial cells, with a cytoplasmic and perinuclear pattern. The observed variability in expression of VEGF and its receptors probably relates to the fact that lymphoma is a heterogeneous lymphoproliferative tumour. Individual differences in VEGF and VEGFR expression must be taken into account when VEGF and VEGFR-targeted approaches for anti-angiogenic therapy are considered in dogs.

Keywords Pubmed: Animals
Dog Diseases/metabolism*
Dog Diseases/pathology
Gene Expression Regulation, Neoplastic
Lymph Nodes/metabolism
Lymph Nodes/pathology
Lymphoma, B-Cell/metabolism
Lymphoma, B-Cell/veterinary*
Lymphoma, T-Cell/metabolism
Lymphoma, T-Cell/veterinary*
RNA, Messenger/metabolism
Vascular Endothelial Growth Factor A/metabolism*
Vascular Endothelial Growth Factor Receptor-1/metabolism*
Vascular Endothelial Growth Factor Receptor-2/metabolism*

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