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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2001

Author(s): Düchler, M; Pengg, M; Brunner, S; Müller, M; Brem, G; Wagner, E

Title: Transfection of epithelial cells is enhanced by combined treatment with mannitol and polyethyleneglycol.

Source: J Gene Med. 2001; 3(2):115-124



Authors Vetmeduni Vienna:

Brem Gottfried
Müller Mathias

Vetmed Research Units
Department for Agrobiotechnology (IFA Tulln)
Institute of Animal Breeding and Genetics


Abstract:
Gene transfer efficiency drops significantly when polarized mammary epithelial cells are transfected instead of actively growing cells. However, fully differentiated cells are the targets for gene transfer in many in vivo applications. Therefore, a simple and effective method for the transfection of polarized mammary epithelial cells in confluent monolayers was developed.

Keywords Pubmed: Animals
Caco-2 Cells
Cell Line
DNA/metabolism
Epithelial Cells/drug effects*
Epithelial Cells/metabolism
Humans
K562 Cells
Mannitol/pharmacology*
Particle Size
Polyethylene Glycols/pharmacology*
Sheep
Transfection*


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