University of Veterinary Medicine Vienna - Research portal

Diagrammed Link to Homepage University of Veterinary Medicine, Vienna

Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2001

Authors: Düchler, M; Pengg, M; Brunner, S; Müller, M; Brem, G; Wagner, E

Title: Transfection of epithelial cells is enhanced by combined treatment with mannitol and polyethyleneglycol.

Source: J Gene Med. 2001; 3(2):115-124

Authors Vetmeduni Vienna:

Brem Gottfried
Müller Mathias

Vetmed Research Units
Department for Agrobiotechnology (IFA Tulln)
Institute of Animal Breeding and Genetics

Gene transfer efficiency drops significantly when polarized mammary epithelial cells are transfected instead of actively growing cells. However, fully differentiated cells are the targets for gene transfer in many in vivo applications. Therefore, a simple and effective method for the transfection of polarized mammary epithelial cells in confluent monolayers was developed.

Keywords Pubmed: Animals
Caco-2 Cells
Cell Line
Epithelial Cells/drug effects*
Epithelial Cells/metabolism
K562 Cells
Particle Size
Polyethylene Glycols/pharmacology*

© University of Veterinary Medicine ViennaHelp and Downloads