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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2001

Authors: Nechtelberger, D; Kaltwasser, C; Stur, I; Meyer, JN; Brem, G; Mueller, M; Mueller, S

Title: DNA microsatellite analysis for parentage control in Austrian pigs.

Source: Anim Biotechnol. 2001; 12(2):141-144

Authors Vetmeduni Vienna:

Brem Gottfried
Müller Mathias
Müller Simone
Sommerfeld-Stur Irene

Vetmed Research Units
Institute of Animal Breeding and Genetics

We present an efficient parentage control for pigs based on ten polymorphic microsatellite markers analyzed in a single PCR reaction. Assuming one known parent ("paternity control"), combined exclusion probabilities (CEPs) ranged from 99.18% (Landrace), 99.74% (Piétrain) to 99.76% (Large White) for the most important Austrian breeds. Assuming a known parent-pair ("parentage control", e.g. a substituted offspring), the CEP of the 10-plex PCR increased to 99.97% (Landrace) and 99.99% (Piétrain and Large White). We developed an additional standby battery of 5 markers, which might be applied in those cases, where the CEP of the 10-plex PCR is not sufficient. Therefore an automated, cost and time reduced genotype analysis for pigs is available.

Keywords Pubmed: Animal Husbandry
Microsatellite Repeats*
Polymerase Chain Reaction
Polymorphism, Genetic*

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