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Publication type: Journal Article
Document type: Full Paper

Year: 2001

Author(s): Nowshari, MA; Brem, G

Title: Effect of freezing rate and exposure time to cryoprotectant on the development of mouse pronuclear stage embryos.

Source: Hum Reprod (16), 11 2368-2373.



Authors Vetmeduni Vienna:

Brem Gottfried

Vetmed Research Units
Institute of Animal Breeding and Genetics


Abstract:
The effects of exposure time (20 versus 45 s) to a high concentration of cryoprotectant (7.0 mol/l ethylene glycol with 0.5 mol/l sucrose) and freezing rates (1200-10 300 degrees C/min) during rapid freezing of mouse pronuclear stage embryos on survival and development to blastocysts were investigated. Different freezing rates were achieved by directly plunging the straws (rapid freezing) and open pulled straws (OPS) in liquid nitrogen (OPS freezing) and by plunging the straws (super rapid) and OPS (super OPS) in a super cooled liquid nitrogen chamber (at -212 degrees C) before storage in liquid nitrogen.

Keywords Pubmed: Animals
Blastocyst/physiology
Cryopreservation/instrumentation
Cryopreservation/methods*
Cryoprotective Agents/administration & dosage*
Ethylene Glycol/administration & dosage
Female
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Nitrogen
Sucrose/administration & dosage
Time Factors
Zygote/growth & development*


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