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Type of publication: Journal Article
Type of document: Full Paper

Year: 2002

Authors: Möstl, E; Maggs, JL; Schrötter, G; Besenfelder, U; Palme, R

Title: Measurement of cortisol metabolites in faeces of ruminants.

Source: Vet Res Commun. 2002; 26(2):127-139

Authors Vetmeduni Vienna:

Besenfelder Urban
Möstl Erich
Palme Rupert

Vetmed Research Units
University Clinic for Ruminants, Clinical Unit of Ruminant Medicine
Department for Agrobiotechnology (IFA Tulln)
Institute for Medical Biochemistry
Institute of Animal Breeding and Genetics

Project(s): Non-invasive monitoring of steroid hormone production

Twenty-one metabolites were detected in faecal samples collected after infusion of (14C)cortisol into the jugular vein of sheep. Using high-performance liquid chromatography/radiometric analysis plus mass spectrometry. One group of metabolites had molecular weights of between 302 and 308, and another group of 350, which indicates that the substances have a C19O3 or a C21O4 structure. Therefore, an enzyme immunoassay against 5beta-androstane-3alpha-ol-11,17-dione-17-CMO:BSA was established. Faecal samples were collected from 10 cows immediately after transport and then during a course in which non-invasive diagnostic procedures were being taught (course 1). For comparison, faeces were sampled from another 5 cows that were being used for teaching invasive procedures (course 2). Six cows from a university farm served as controls. In the animals used in course 1, the highest concentrations of cortisol metabolites were measured immediately after transport to the university (median value: 2.2 micromol/kg faeces). During the first 5 days at the university, the concentrations decreased to 0.52 micromol/kg (median) and remained at this level during the rest of the course. The median concentration in the samples that were taken during coursc 2 (collected about 2 months after transport) was 0.48 micromol/kg. There was no significant difference in the excretion of cortisol metabolites between these cows and the controls. We conclude from these data that, using the enzyme immunoassay against 5beta-androstane-3alpha-ol-11,17-dione-17-CMO, we were able to detect transport/novel environment stress but not the potential disturbance that cows experience during diagnostic procedures.

Keywords Pubmed: Animals
Carbon Radioisotopes/metabolism*
Chromatography, Liquid
Immunoenzyme Techniques
Logistic Models
Mass Spectrometry
Stress, Physiological/diagnosis
Stress, Physiological/metabolism
Time Factors

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