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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2002

Authors: Huber, S; Bruns, U; Arnold, W

Title: Sex determination of red deer using polymerase chain reaction of DNA from feces.

Source: Wildlife Society Bulletin (30) 208-212.

Authors Vetmeduni Vienna:

Arnold Walter
Bruns Ute
Huber Susanne

Vetmed Research Units
Institute of Animal Breeding and Genetics
Research Institute of Wildlife Ecology


Abstract:
We describe a method to determine the sex of red deer (Cervus elaphus) from feces. A modified extraction protocol for fecal DNA, together with a duplex polymerase chain reaction (PCR) amplifying the sex-determining region Y gone (Sry) and an autosomal microsatellite locus, yielded the correct sex in fecal samples from 11 known animals. With repeated analysis of 40 feces from unknown individuals, we obtained identical results in 85% of all replicates. Sexing of fecal samples enhances the potential value of analyses from feces, thereby increasing the effectiveness of these tools in field studies and applied research.


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