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Publication type: Journal Article
Document type: Full Paper

Year: 2002

Author(s): Huarte, E; Sarobe, P; Lasarte, JJ; Brem, G; Weiss, EH; Prieto, J; BorrĂ¡s-Cuesta, F

Title: Identification of HLA-B27-restricted cytotoxic T lymphocyte epitope from carcinoembryonic antigen.

Source: Int J Cancer. 2002; 97(1):58-63



Authors Vetmeduni Vienna:

Brem Gottfried

Vetmed Research Units
Institute of Animal Breeding and Genetics


Abstract:
Characterization of epitopes recognized by cytotoxic T lymphocytes (CTLs) in the sequence of tumor antigens is an important step in the development of tumor therapies. Because carcinoembryonic antigen (CEA) is a protein expressed in a high number of epithelial tumors, it is an interesting target to study. We screened for the presence of HLA-B27-restricted CTL epitopes from CEA by studying the binding to HLA-B27 of 31 synthetic peptides predicted to bind to this molecule. This afforded 16 peptides with moderate or high binding affinity. Immunization of HLA-B27 transgenic mice with the best binder peptides yielded 4 immunogenic peptides: CEA(9-17), CEA(9-18), CEA(138-146) and CEA(360-369). However, splenocytes from mice immunized with a vaccinia virus-expressing CEA recognized only CEA(9-18). These CTLs were of the CD8(+) phenotype, which upon stimulation with peptide specifically produced IFN-gamma. Moreover, they did not cross-react against peptides of region 9-18 from proteins of the CEA family. Our results show that CEA(9-18) may induce specific CTL responses against CEA.

Keywords Pubmed: Animals
Binding Sites
Carcinoembryonic Antigen/genetics
Carcinoembryonic Antigen/immunology*
Cell Line
Cross Reactions
Epitopes, T-Lymphocyte/immunology*
HLA-B27 Antigen/immunology*
Humans
Immunization
Interferon-gamma/metabolism
Mice
Mice, Transgenic
Peptides/chemistry
Peptides/immunology*
T-Lymphocytes, Cytotoxic/immunology*
Vaccinia virus
beta 2-Microglobulin/genetics
beta 2-Microglobulin/immunology*


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