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Type of publication: Journal Article
Type of document: Full Paper

Year: 2003

Authors: Jauk, V; Neubauer, C; Szölgyényi, W; Vasicek, L

Title: Phenotypic and genotypic differentiation of Campylobacter spp. isolated from Austrian broiler farms: a comparison.

Source: Avian Pathol. 2003; 32(1):33-37

Authors Vetmeduni Vienna:

Hess Claudia
Vasicek Lore

Vetmed Research Units
University Clinic for Poultry and Fish Medicine

An identification scheme based on restriction fragment length polymorphism of polymerase chain reaction products (PCR-RFLP) was developed to differentiate isolates of the genera Campylobacter, Arcobacter and Helicobacter. Based on the 16S rRNA gene of these genera, PCR amplified a 1216-bp fragment. The amplicons were digested with the restriction enzymes RsaI and EcoRV. Additional differentiation was obtained using a PCR-assay based on the hippuricase gene. Genotyping was performed on several reference strains from the National Collection of Typing Culture (NCTC), London, and on 130 field isolates. In parallel, a phenotypic differentiation was performed, in order to compare the results. In 119 cases (91.5%) the results obtained from the genotypic characterization were concordant with those from phenotypic testing. Co-infections with Campylobacter jejuni and Campylobacter coli in two samples and seven hippurate-negative C. jejuni-strains were identified by the genotypic method. Furthermore, PCR-RFLP assays identified an atypical isolate as Campylobacter fetus/hyointestinalis.

Keywords Pubmed: Animals
Campylobacter/isolation & purification*
Campylobacter Infections/epidemiology
Campylobacter Infections/microbiology*
Campylobacter Infections/veterinary*
Polymorphism, Restriction Fragment Length
Poultry Diseases/epidemiology
Poultry Diseases/microbiology*
RNA, Ribosomal, 16S/genetics

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