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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2003

Authors: Huber, S; Bruns, U; Arnold, W

Title: Genotyping herbivore feces facilitating their further analyses.

Source: Wildlife Society Bulletin (31), 3 692-697.


Authors Vetmeduni Vienna:

Arnold Walter
Bruns Ute
Huber Susanne

Vetmed Research Units
Institute of Animal Breeding and Genetics
Research Institute of Wildlife Ecology


Project(s): Stress and bark peeling in red deer


Abstract:
We examined the utility of a DNA extraction protocol that proved to be useful for sex determination from feces for non-invasive genotyping of red deer (Cervus elaphus) and European hare (Lepus europaeus) feces. The exceptional feature of this method is that it leaves the genotyped feces intact for further analyses. This may be important for studies combining genetic with other fecal examinations where the amount of fecal material is limited. Allele patterns from matched blood and fecal DNA extracts were identical in only 80% of repeated fecal polymerase chain reaction (PCR) amplifications. Repetitions may increase reliability of results but also increase time and money expended for genotyping. Before using microsatellites in feces, advantages and disadvantages of various DNA extraction protocols and of the use of feces as sample material should be evaluated.


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