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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2003

Authors: Hilbert, F; Mayrhofer, S; Smulders, FJ

Title: Rapid urease screening of Yersinia on CIN agar plates.

Source: Int J Food Microbiol. 2003; 84(1):111-115

Authors Vetmeduni Vienna:

Hilbert Friederike
Smulders Franciscus

Vetmed Research Units
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Food Hygiene and Technology

Yersinia enterocolitica is an important foodborne pathogen, but isolation of virulent Yersinia from food sources is still time consuming and requires skills. In this article, we describe a rapid urease screening on cefsulodin-irgasan-novobiocin (CIN) agar plates with an agar overlay assay. This test is simple to perform, all colonies on a plate can be checked simultaneously, it only takes minutes for detection of urease-positive colonies and the colonies survive for transfer, further characterisation, and storage. Additionally, this method is useful to isolate virulent (urease-positive and pYV harbouring) Y. enterocolitica from foodstuffs.

Keywords Pubmed: Agar
Culture Media*
Enzyme Inhibitors/metabolism
Time Factors
Yersinia enterocolitica/enzymology*
Yersinia enterocolitica/isolation & purification

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