University of Veterinary Medicine Vienna - Research portal

Diagrammed Link to Homepage University of Veterinary Medicine, Vienna

Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2003

Authors: Hilbert, F; Mayrhofer, S; Smulders, FJ

Title: Rapid urease screening of Yersinia on CIN agar plates.

Source: Int J Food Microbiol. 2003; 84(1):111-115



Authors Vetmeduni Vienna:

Hilbert Friederike
Smulders Franciscus

Vetmed Research Units
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Food Hygiene and Technology


Abstract:
Yersinia enterocolitica is an important foodborne pathogen, but isolation of virulent Yersinia from food sources is still time consuming and requires skills. In this article, we describe a rapid urease screening on cefsulodin-irgasan-novobiocin (CIN) agar plates with an agar overlay assay. This test is simple to perform, all colonies on a plate can be checked simultaneously, it only takes minutes for detection of urease-positive colonies and the colonies survive for transfer, further characterisation, and storage. Additionally, this method is useful to isolate virulent (urease-positive and pYV harbouring) Y. enterocolitica from foodstuffs.

Keywords Pubmed: Agar
Carbanilides/metabolism
Cefsulodin/metabolism
Culture Media*
Enzyme Inhibitors/metabolism
Novobiocin/metabolism
Time Factors
Urease/metabolism*
Yersinia enterocolitica/enzymology*
Yersinia enterocolitica/isolation & purification


© University of Veterinary Medicine ViennaHelp and DownloadsAccessibility statement