Yersinia enterocolitica is an important foodborne pathogen, but isolation of virulent Yersinia from food sources is still time consuming and requires skills. In this article, we describe a rapid urease screening on cefsulodin-irgasan-novobiocin (CIN) agar plates with an agar overlay assay. This test is simple to perform, all colonies on a plate can be checked simultaneously, it only takes minutes for detection of urease-positive colonies and the colonies survive for transfer, further characterisation, and storage. Additionally, this method is useful to isolate virulent (urease-positive and pYV harbouring) Y. enterocolitica from foodstuffs.
Agar Carbanilides/metabolism Cefsulodin/metabolism Culture Media* Enzyme Inhibitors/metabolism Novobiocin/metabolism Time Factors Urease/metabolism* Yersinia enterocolitica/enzymology* Yersinia enterocolitica/isolation & purification