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Publication type: Journal Article
Document type: Full Paper

Year: 2003

Author(s): Kapustin, DV; Iagudaeva, EIu; Zavada, LL; Zhigis, LS; Zubov, VP; Iaroshevskaia, EM; Plobner, L; Leiser, RM; Brem, G

Title: [A composite polyaniline-containing silica sorbent for DNA isolation].

Source: Bioorg Khim. 2003; 29(3):310-315

Authors Vetmeduni Vienna:

Brem Gottfried

Vetmed Research Units
Institute of Animal Breeding and Genetics

A composite sorbent based on porous glass beads modified with thin polyaniline coating was prepared by precipitating aniline polymerization in the presence of carrier particles. It was shown that the modification ensures the uniform coating of the inner surface of the carrier pores with the polymer layer approximately 70 A thick. It was shown that the resulting material retains the initial porosity of the carrier and is selective in the separation of nucleic acids and proteins. The polyaniline-coated sorbents were shown to be efficient for both the preparative DNA isolation from bacterial lysates and for analytical purposes, in particular, for studying DNA fragmentation during apoptosis proceeding under UV irradiation of cell lysates of colon carcinoma. The morphological and chromatographic characteristics of the new sorbent were demonstrated to be similar to those of the polyfluorobutadiene sorbent.

Keywords Pubmed: Aniline Compounds/chemistry*
Apoptosis/radiation effects
Colonic Neoplasms/genetics
Colonic Neoplasms/pathology
DNA/isolation & purification*
DNA, Bacterial/isolation & purification
DNA, Neoplasm/isolation & purification
Electrophoresis, Polyacrylamide Gel
Escherichia coli/genetics
Silicon Dioxide/chemistry*
Ultraviolet Rays

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