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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 1999

Author(s): Aigner, B; Fleischmann, M; Muller, M; Brem, G

Title: Stable long term germ-line transmission of transgene integration sites in mice.

Source: Transgenic Res 1999; 8(1): 1-8.



Authors Vetmeduni Vienna:

Aigner Bernhard
Brem Gottfried
Müller Mathias

Vetmed Research Units
Institute of Animal Breeding and Genetics


Abstract:
Transgenic mice provide a valuable tool in all fields of basic and applied biological and medical research. In this study, we describe the fate of integrated transgenes in the mammalian host genome over a large number of generations. The stability of the germ-line transmission of integrated tyrosinase transgene copies was monitored up to generation F20 in a large number of individuals from seven transgenic mouse lines. Phenotypic and molecular genetic analysis of the offspring both within the different lines and in cross-breeding experiments revealed the high stability of the transgene integration sites in mice. Only very few individuals were affected by a transgene copy loss. These results indicate that, once homozygous transgenic lines are established, breeding programs can be continued to a high number of generations without further stringent molecular genetic analysis.


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