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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2000

Author(s): Reichart, U; Kappler, R; Scherthan, H; Wolf, E; Müller, M; Brem, G; Aigner, B

Title: Partial leptin receptor gene deletion in transgenic mice prevents expression of the membrane-bound isoforms except for Ob-Rc.

Source: Biochem Biophys Res Commun. 2000; 269(2):496-501



Authors Vetmeduni Vienna:

Aigner Bernhard
Brem Gottfried
Müller Mathias
Reichart Ursula

Vetmed Research Units
Institute of Animal Breeding and Genetics


Abstract:
Examination of random insertional mutations in transgenic animals harbouring an abnormal phenotype contributes to the discovery of new genes and/or the understanding of already known genes. Here we describe a transgenic mouse line showing early-onset obesity as consequence of the transgene insertion. Molecular genetic analysis revealed a partial deletion of the leptin receptor (Lepr, Ob-R) gene including the coding sequences downstream of exon 17". This defect prevents the expression of all described membrane-bound isoforms of Ob-R except for isoform Ob-Rc in the homozygous transgenic animals. Thus, this mouse model might be useful for the investigation of the function of the short Ob-R isoforms.

Keywords Pubmed: Animals
Carrier Proteins/genetics*
Gene Deletion*
Leptin/genetics*
Mice
Mice, Transgenic
Mutagenesis, Insertional
Protein Isoforms/genetics*
RNA, Messenger/genetics
Receptors, Cell Surface*
Receptors, Leptin
Transgenes


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