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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2008

Author(s): Käser, T; Gerner, W; Hammer, SE; Patzl, M; Saalmüller, A

Title: Phenotypic and functional characterisation of porcine CD4(+)CD25(high) regulatory T cells.

Source: Vet Immunol Immunopathol (122), 1-2 153-158.



Authors Vetmeduni Vienna:

Gerner Wilhelm
Hammer Sabina
Käser Tobias
Patzl Martina
Saalmüller Armin

Vetmed Research Units
Institute of Immunology


Abstract:
Over the last years regulatory T cells (Tregs) were defined as CD4(+)CD25(+) T lymphocytes expressing the transcription factor Foxp3 (Forkhead Box P3) with the ability to downregulate various immune responses. In swine the existence of CD4(+)CD25(+) T lymphocytes was described before but nothing is known about the function of this minor cell population to date. Therefore, we studied porcine CD4(+)CD25(+) T cells with regard to major attributes of murine and human Tregs: their phenotype concerning the expression of several T-cell specific antigens, including Foxp3, their IL-10 production and their suppressive capacity. Our results revealed that porcine CD4(+)CD25(+) T cells with high CD25 expression count for about 2-9% of the CD4(+) T-cell subset. They demonstrate a strong Foxp3 expression, a heterogeneous CD45RC-, CD8alpha- and MHC-II-defined phenotype and a moderate IL-10 production. Co-cultivation of increasing numbers of CD4(+)CD25(high) T cells with a constant number of CD4(+)CD25(-) responder-T cells caused a decrease in proliferation of the entire culture. This demonstrates the suppressive capacity of the CD4(+)CD25(high) T-cell subset and - together with their Foxp3 expression - the existence of porcine Tregs.

Keywords Pubmed: Animals
Forkhead Transcription Factors/analysis
Immunophenotyping
Interleukin-10/biosynthesis
Swine/immunology*
T-Lymphocytes, Regulatory/immunology*
T-Lymphocytes, Regulatory/physiology


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