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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2004

Authors: Schaschl, H; Goodman, SJ; Suchentrunk, F

Title: Sequence analysis of the MHC class II DRB alleles in Alpine chamois (Rupicapra r. rupicapra).

Source: Dev Comp Immunol. 2004; 28(3):265-277

Authors Vetmeduni Vienna:

Suchentrunk Franz

Vetmed Research Units
Research Institute of Wildlife Ecology

This study characterises the expressed MHC class II DRB gene and its genetic variation in exon 2 among 59 Alpine chamois (Rupicapra r. rupicapra) from several locations in the Eastern Alps plus one population in the Western Alps. The expressed DRB gene contains all the expected characteristics including all of the conserved residues found in other mammalian species. The isolated expressed DRB gene from chamois can be considered orthologous to MHC class II DRB genes in other mammalian species. Cattle primers yielded exon 2 sequences of 284 bp length. Sequence analysis revealed 19 DRB exon 2 alleles among 59 chamois. Alleles exhibited a high degree of nucleotide and amino acid polymorphism, with most amino acid variations occurring at positions forming the peptide-binding sites. A significantly higher rate of non-synonymous (dN = 0.053+/-0.0132) than synonymous (dS = 0.007+/-0.003) substitutions indicated positive selection for diversity in the DRB locus.

Keywords Pubmed: Amino Acid Sequence
Amino Acid Substitution/genetics
Binding Sites/genetics
Cloning, Molecular
DNA, Complementary/chemistry
DNA, Complementary/genetics
Gene Frequency
Genes, MHC Class II/genetics*
Histocompatibility Antigens Class II/genetics*
Molecular Sequence Data
Polymorphism, Genetic/genetics
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid

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