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Type of publication: Journal Article
Type of document: Full Paper

Year: 2009

Authors: Gregersen, RH; Neubauer, C; Christensen, H; Bojesen, AM; Hess, M; Bisgaard, M

Title: Comparative studies on [Pasteurella] testudinis and [P.] testudinis-like bacteria and proposal of Chelonobacter oris gen. nov., sp. nov. as a new member of the family Pasteurellaceae.

Source: Int J Syst Evol Microbiol. 2009; 59(Pt 7):1583-1588



Authors Vetmeduni Vienna:

Hess Claudia
Hess Michael

Vetmed Research Units
University Clinic for Poultry and Fish Medicine


Abstract:
A collection of 12 strains, isolated from diseased tortoises and tentatively identified as [Pasteurella] testudinis-like based on phenotypic characters, was compared with three reference strains of [P.] testudinis. All strains could be separated from the reference strains with respect to 16S rRNA gene sequences, partial sequences of the rpoB housekeeping gene and by phenotypic characters. Based upon differences in 16S rRNA and rpoB gene sequences, the new isolates are suggested to represent a novel species in a new genus of the family Pasteurellaceae Pohl 1981, for which the name Chelonobacter oris gen. nov., sp. nov. is proposed. The type strain is 1662(T) (=CCUG 55632(T)=DSM 21392(T)). beta-Haemolysis and acid production from (+)-l-arabinose, dulcitol, (-)-d-mannitol, (+)-d-mannose, trehalose and salicin separated the new strains from members of existing genera of the family Pasteurellaceae, in addition to the beta-galactosidase, urease and alpha-glucosidase reactions. Differences in indole production, phosphatase, beta-glucosidase and production of acid from dulcitol and trehalose separated C. oris from [P.] testudinis. Several phenotypic characters separated C. oris from Bisgaard"s taxa 14 and 32.

Keywords Pubmed: Animals
Bacterial Typing Techniques
DNA, Bacterial/analysis
DNA-Directed RNA Polymerases/genetics
Genes, rRNA
Genotype
Molecular Sequence Data
Pasteurella/classification*
Pasteurella/genetics
Pasteurella/isolation & purification
Pasteurella/physiology
Pasteurella Infections/microbiology
Pasteurella Infections/veterinary*
Pasteurellaceae/classification*
Pasteurellaceae/genetics
Pasteurellaceae/physiology
Phenotype
Phylogeny
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Species Specificity
Turtles/microbiology*


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