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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 1999

Authors: Coulibaly, S; Besenfelder, U; Fleischmann, M; Zinovieva, N; Grossmann, A; Wozny, M; Bartke, I; Tögel, M; Müller, M; Brem, G

Title: Human nerve growth factor beta (hNGF-beta): mammary gland specific expression and production in transgenic rabbits.

Source: FEBS Lett (444), 1 111-116.



Authors Vetmeduni Vienna:

Besenfelder Urban
Brem Gottfried
Müller Mathias

Vetmed Research Units
Institute of Animal Breeding and Genetics


Abstract:
Transgenic rabbits carrying gene constructs encoding human nerve growth factor beta (hNGF-beta) cDNA were generated. Expression of hNGF-beta mRNA was restricted to the mammary gland of lactating rabbits. Western Blot analysis revealed a polypeptide of 13.2 kDa in the milk of transgenic animals. hNGF-beta was purified from the milk by a two-step chromatographic procedure. Electrospray mass spectroscopy analysis of purified hNGF-beta depicted a molecular weight of 13,261 Da per subunit. The biological activity of the hNGF-beta was tested using PC12W2 cells and cultures of dorsal root ganglion neurons from chicken embryos. Crude defatted milk from transgenic animals and purified hNGF-beta demonstrated full biological activity when compared to commercial recombinant hNGF-beta.

Keywords Pubmed: Animals
Animals, Genetically Modified
Cell Size/drug effects
Cells, Cultured
Chick Embryo
Dose-Response Relationship, Drug
Female
Ganglia, Spinal/cytology
Humans
Lactation/metabolism
Male
Mammary Glands, Animal/metabolism*
Mass Spectrometry
Milk/metabolism
Nerve Growth Factors/chemistry
Nerve Growth Factors/genetics
Nerve Growth Factors/metabolism*
Nerve Growth Factors/pharmacology
Neurites/drug effects
Neurites/metabolism
Organ Specificity
PC12 Cells
RNA, Messenger/analysis
Rabbits
Rats
Recombinant Proteins/chemistry
Recombinant Proteins/genetics
Recombinant Proteins/isolation & purification
Recombinant Proteins/pharmacology


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