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Publication type: Journal Article
Document type: Full Paper

Year: 1998

Author(s): Durcova-Hills, G; Prelle, K; Müller, S; Stojkovic, M; Motlik, J; Wolf, E; Brem, G

Title: Primary culture of porcine PGCs requires LIF and porcine membrane-bound stem cell factor.

Source: Zygote (6), 3 271-275.

Authors Vetmeduni Vienna:

Brem Gottfried
Müller Simone

Vetmed Research Units
Institute of Animal Breeding and Genetics

We studied the effect of murine leukaemia inhibitory factor (LIF), human basic fibroblast growth factor (bFGF) and porcine stem cell factor (SCF) on the survival and/or proliferation of porcine primordial germ cells (PGCs) obtained from 27-day-old embryos in vitro. PGCs were cultured in embryonic stem cell (ESC) medium supplemented with or without either LIF (1000 IU/ml) alone or LIF together with bFGF (10 ng/ml). They were seeded on mitotically inactivated feeder cells, either STO or transfected STO cells (STO#8), expressing the membrane-bound form of porcine SCF. PGCs were identified by their alkaline phosphatase (AP) activity and counted after 1, Sand 5 days in culture. After 1 day of culture, PGCs cultured on STO#8 cells showed significantly higher survival than PGCs cultured on STO cells (p < 0.05). The combined effect of SCF and UF caused a significant increase in PGC number by day 3 of culture when PGCs were cultured on either STO cells (p < 0.01) or STO#8 (p < 0.001). When SCT: and LIF were used together with bFGF no increase in the PGC number was observed. Our results suggest that the membrane-bound form of porcine SCF plays a pivotal role in the primary culture of porcine PGCs and that bFGF is not required. in vitro.

Keywords Pubmed: Alkaline Phosphatase - metabolism : Animals - metabolism : Cell Culture Techniques - methods : Cell Division - drug effects : Cell Survival - drug effects : Embryo, Mammalian - metabolism : Fibroblast Growth Factor 2 - pharmacology : Germ Cells - metabolism : Growth Inhibitors - pharmacology : Growth Substances - pharmacology : Histocytochemistry - pharmacology : Humans - pharmacology : Interleukin-6 - pharmacology : Leukemia Inhibitory Factor - pharmacology : Lymphokines - pharmacology : Membrane Proteins - metabolism : Stem Cell Factor - pharmacology : Swine - pharmacology

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