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Publication type: Journal Article
Document type: Full Paper

Year: 1996

Author(s): Van Langendonckt, A; Vansteenbrugge, A; Donnay, I; Van Soom, A; Berg, U; Semple, E; Grisart, B; Mermillod, P; Brem, G; Massip, A; Dessy, F

Title: Three year results of in vitro production of bovine embryos in serum-poor bovine oviduct conditioned medium. An overview.

Source: Reprod Nutr Dev (36), 5 493-502.



Authors Vetmeduni Vienna:

Brem Gottfried

Vetmed Research Units
Institute of Animal Breeding and Genetics


Abstract:
This paper presents a synthesis of 3 year results of in vitro production of bovine embryos in medium previously conditioned by bovine oviduct epithelial cells. In Louvain-la-Neuve, Belgium, a total of 18 356 oocytes were matured and inseminated in vitro: 13 967 (76%) had cleaved at 3 days postinsemination and 3 593 (26%) became blastocysts using this culture system. Our data show that conditioned medium can be stored frozen for up to 3 years without significant loss of activity and is resistant to lyophilization. One single batch of conditioned medium was tested within the same period in four different laboratories and yielded variable results: 27 and 37% blastocysts/cleaved embryos in two of them and only 7 and 0% in the two others whereas in each case more than 30% blastocysts were obtained with the local reference co-culture system. In one laboratory, the batch of oil used to overlay the culture drops had a detrimental effect on the blastocyst rate in conditioned medium but not in coculture.

Keywords Pubmed: Animals - : Cattle - : Culture Media, Conditioned - : Culture Media, Serum-Free - : Culture Techniques - : Embryonic and Fetal Development - : Fallopian Tubes - metabolism : Female - metabolism : Fertilization in Vitro - veterinary : Freeze Drying - veterinary : Freezing - veterinary : Pregnancy - veterinary

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