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Publication type: Journal Article
Document type: Full Paper

Year: 1995

Author(s): Stojkovic, M; Wolf, E; B├╝ttner, M; Berg, U; Charpigny, G; Schmitt, A; Brem, G

Title: Secretion of biologically active interferon tau by in vitro-derived bovine trophoblastic tissue.

Source: Biol Reprod (53), 6 1500-1507.

Authors Vetmeduni Vienna:

Brem Gottfried

Vetmed Research Units
Institute of Animal Breeding and Genetics

Secretion of interferon tau (IFN tau) by trophoblastic tissue has been shown to be the first embryonic signal for pregnancy recognition, Therefore we tried to derive biologically active trophoblastic tissue by in vitro techniques. Since conventional in vitro conditions for bovine embryo development were not sufficient for long-term culture, we tested more complex culture conditions, including Menezo B2 or Buffalo rat liver (BRL) cell-conditioned medium, for their ability to support proliferation and IFN tau secretion by in vitro-derived trophoblastic tissue. IFN tau activity was determined by using a biological assay based on the inhibition of the cytopathic effect of vesicular stomatitis virus on Madin-Darby bovine kidney cells. When cultures of individual hatched blastocysts were started in 60-mu l drops of BRL cell-conditioned medium, mean IFN tau secretion (antiviral units/ml/48 h) corresponded to 1200 on Day 11 and to 5000 on Day 13 (p < 0.01). To characterize trophoblast cell-specific secretions, the inner cell mass was removed from all embryos by microsurgery on Day 13. IFN tau secretion by trophoblastic tissue increased to mean levels of > 10(5) antiviral units/ml/48 h on Day 23, stayed high for about 1 wk, and then slowly declined to levels below 10(3) antiviral U/ml/48 h. The specificity of the cytoprotective effect of IFN tau was tested by Western blot analysis and by immunoneutralization with use of a polyclonal antiserum specific to IFN tau. Our results demonstrate that viable trophoblastic tissue can be maintained entirely in vitro and secretes high amounts of IFN tau.

Keywords Pubmed: Animals - : Blastocyst - physiology : Blotting, Western - physiology : Cattle - physiology : Culture Media, Conditioned - physiology : Culture Techniques - physiology : Female - physiology : Interferon Type I - secretion : Pregnancy - secretion : Pregnancy Proteins - secretion : Time Factors - secretion : Trophoblasts - secretion

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