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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 1998

Authors: Fleischmann, M; Bloch, W; Kolossov, E; Andressen, C; Müller, M; Brem, G; Hescheler, J; Addicks, K; Fleischmann, BK

Title: Cardiac specific expression of the green fluorescent protein during early murine embryonic development.

Source: FEBS Lett (440), 3 370-376.



Authors Vetmeduni Vienna:

Brem Gottfried
Müller Mathias

Vetmed Research Units
Institute of Animal Breeding and Genetics


Abstract:
We demonstrate the establishment of transgenic mice, where the expression of the green fluorescent protein (GFP) is under control of the human cardiac alpha-actin promoter. These mice display cardiac specific GFP expression already during early embryonic development. Prominent GFP fluorescence was observed at the earliest stage of the murine heart anlage (E8). Cardiomyocytes of different developmental stages proved GFP positive, but the intensity varied between cells. We further show that contractions of single GFP positive cardiomyocytes can be monitored within the intact embryo. At later stages of embryonic development, the skeletal musculature was also GFP positive, in line with the known expression pattern of cardiac alpha-actin. The tissue specific labeling of organs is a powerful new tool for embryological as well as functional investigations in vivo.

Keywords Pubmed: Actins/genetics
Animals
Female
Gene Expression Regulation
Genes, Reporter
Green Fluorescent Proteins
Heart/embryology*
Heart/physiology
Humans
Luminescent Proteins/biosynthesis
Luminescent Proteins/genetics*
Male
Mice
Mice, Transgenic
Muscle, Skeletal/metabolism
Myocardium/metabolism*
Promoter Regions, Genetic


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