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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2010

Authors: Klinglmayr, C; Nöbauer, K; Razzazi-Fazeli, E; Cichna-Markl, M

Title: Determination of deoxynivalenol in organic and conventional food and feed by sol-gel immunoaffinity chromatography and HPLC-UV detection.

Source: J Chromatogr B Analyt Technol Biomed Life Sci. 2010; 878(2):187-193

Authors Vetmeduni Vienna:

Nöbauer Katharina
Razzazi-Fazeli Ebrahim

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds

The paper describes the determination of deoxynivalenol (DON) in 55 wheat food and feed samples, 26 from conventional and 29 from organic production. Immunoaffinity columns prepared by entrapping anti-DON antibodies by the sol-gel method were used for sample clean-up. DON was quantified by high performance liquid chromatography (HPLC) and ultraviolet (UV) detection. In general, the incidence of DON contamination was rather low. In eight samples (14.5%) the DON concentration was above the LOQ (380ng/g), in six samples (10.9%) DON was detected but could not be quantified (>LOD (200ng/g), LOQ. The data indicate both a higher incidence of DON contamination and higher DON concentrations in food and feed samples from conventional than in those from organic production.

Keywords Pubmed: Animal Feed/analysis
Chromatography, Affinity/methods*
Chromatography, High Pressure Liquid/methods*
Food Analysis/methods*
Health Food/analysis
Immunosorbent Techniques*
Phase Transition
Spectrophotometry, Ultraviolet

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