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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2011

Authors: Gutiérrez, AM; Miller, I; Hummel, K; Nöbauer, K; Martínez-Subiela, S; Razzazi-Fazeli, E; Gemeiner, M; Cerón, JJ

Title: Proteomic analysis of porcine saliva.

Source: Vet J. 2011; 187(3):356-362



Authors Vetmeduni Vienna:

Gemeiner Manfred
Hummel Karin
Miller Ingrid
Nöbauer Katharina
Razzazi-Fazeli Ebrahim

Vetmed Research Units
Institute for Medical Biochemistry
VetCore


Abstract:
Saliva contains a number of proteins that may be useful as biomarkers of health and disease and can be easily obtained from large numbers of animals in a non-invasive, stress-free way. The objective of this study was to explore the protein composition of porcine saliva from 10 specific pathogen free pigs using first one-dimensional SDS-PAGE and then two-dimensional electrophoresis and mass spectrometry. A reference proteome pattern for porcine saliva was established with the identification of 13 different, mainly saliva-specific, proteins. These reference data will facilitate the investigation of salivary proteins potentially altered in disease and could serve as novel diagnostic biomarkers.

Keywords Pubmed: Animals
Biological Markers/analysis
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel/veterinary
Male
Mass Spectrometry/veterinary
Proteome/chemistry*
Proteome/isolation & purification
Saliva/chemistry*
Salivary Proteins and Peptides/chemistry*
Salivary Proteins and Peptides/isolation & purification
Specific Pathogen-Free Organisms
Swine
Swine Diseases/diagnosis


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