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Type of publication: Journal Article
Type of document: Full Paper

Year: 2010

Authors: Butschi, A; Titz, A; Wälti, MA; Olieric, V; Paschinger, K; Nöbauer, K; Guo, X; Seeberger, PH; Wilson, IB; Aebi, M; Hengartner, MO; Künzler, M

Title: Caenorhabditis elegans N-glycan core beta-galactoside confers sensitivity towards nematotoxic fungal galectin CGL2.

Source: PLoS Pathog. 2010; 6(1):e1000717



Authors Vetmeduni Vienna:

Nöbauer Katharina

Vetmed Research Units
VetCore


Abstract:
The physiological role of fungal galectins has remained elusive. Here, we show that feeding of a mushroom galectin, Coprinopsis cinerea CGL2, to Caenorhabditis elegans inhibited development and reproduction and ultimately resulted in killing of this nematode. The lack of toxicity of a carbohydrate-binding defective CGL2 variant and the resistance of a C. elegans mutant defective in GDP-fucose biosynthesis suggested that CGL2-mediated nematotoxicity depends on the interaction between the galectin and a fucose-containing glycoconjugate. A screen for CGL2-resistant worm mutants identified this glycoconjugate as a Galbeta1,4Fucalpha1,6 modification of C. elegans N-glycan cores. Analysis of N-glycan structures in wild type and CGL2-resistant nematodes confirmed this finding and allowed the identification of a novel putative glycosyltransferase required for the biosynthesis of this glycoepitope. The X-ray crystal structure of a complex between CGL2 and the Galbeta1,4Fucalpha1,6GlcNAc trisaccharide at 1.5 A resolution revealed the biophysical basis for this interaction. Our results suggest that fungal galectins play a role in the defense of fungi against predators by binding to specific glycoconjugates of these organisms.

Keywords Pubmed: Agaricales/immunology*
Amino Acid Sequence
Animals
Caenorhabditis elegans/genetics
Caenorhabditis elegans/immunology
Caenorhabditis elegans/metabolism
Caenorhabditis elegans Proteins/genetics
Caenorhabditis elegans Proteins/immunology
Caenorhabditis elegans Proteins/metabolism*
Fungal Proteins/chemistry
Fungal Proteins/immunology*
Fungal Proteins/metabolism
Galactosides/metabolism*
Galectin 2/chemistry
Galectin 2/immunology*
Galectin 2/metabolism
Molecular Sequence Data
Nematode Infections/immunology*
Nematode Infections/metabolism
Protein Structure, Quaternary
Structure-Activity Relationship


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