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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2010

Author(s): Rütgen, BC; Hammer, SE; Gerner, W; Christian, M; de Arespacochaga, AG; Willmann, M; Kleiter, M; Schwendenwein, I; Saalmüller, A

Title: Establishment and characterization of a novel canine B-cell line derived from a spontaneously occurring diffuse large cell lymphoma.

Source: Leuk Res. 2010; 34(7):932-938



Authors Vetmeduni Vienna:

Christian Maria
Gerner Wilhelm
Guija-De-Arespacochaga Abigail
Hammer Sabina
Kleiter Miriam
Rütgen Barbara
Saalmüller Armin
Schwendenwein Ilse
Willmann Michael

Vetmed Research Units
Clinical Pathology Platform
University Clinic for Small Animals, Clinical Unit of Internal Medicine Small Animals
Institute of Immunology


Abstract:
Cell lines derived from spontaneous tumors serve as a research tool for cancer cell biology and new anti-cancer drug development. Isolation and propagation of canine lymphoma cell lines is difficult, thus only a few are available. Now we have established a new B-cell lymphoma cell line CLBL-1 from a dog with confirmed stage IV diffuse large cell lymphoma. Immunophenotyping of these CLBL-1 cells showed positive staining for CD11a, CD79alphacy, CD45, CD45RA, MHC II and cells were negative for CD3, CD4, CD5, CD8, CD11d, CD14, CD21, CD34, CD56 and T-cell receptor-gammadelta (TCR-gammadelta). PCR analysis for TCR-gamma and immunoglobulin heavy chain (IgH) gene rearrangements yielded a monoclonal result for the IgH gene. Furthermore, the clonality of IgH gene rearrangement was confirmed by sequencing of 16 positive bacterial clones. As canine lymphoma resembles non-Hodgkin"s lymphoma (NHL) in humans in many respects, this new cell line, will promote translational and comparative lymphoma research in humans and dogs.

Keywords Pubmed: Animals
Antigens, CD/analysis
Antigens, Neoplasm/analysis
B-Lymphocytes/chemistry
B-Lymphocytes/pathology*
Biopsy, Fine-Needle
Cell Line, Tumor/chemistry
Cell Line, Tumor/pathology*
Cell Separation
Clone Cells/chemistry
Clone Cells/pathology
Dog Diseases/pathology*
Dogs
Flow Cytometry
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Immunophenotyping
Lymph Nodes/pathology
Lymphoma, Large B-Cell, Diffuse/pathology
Lymphoma, Large B-Cell, Diffuse/veterinary*
Male
Neoplasm Proteins/analysis
Receptors, Antigen, T-Cell, gamma-delta/analysis


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