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Type of publication: Journal Article
Type of document: Full Paper

Year: 2009

Authors: Smorodchenko, A; Rupprecht, A; Sarilova, I; Ninnemann, O; Bräuer, AU; Franke, K; Schumacher, S; Techritz, S; Nitsch, R; Schuelke, M; Pohl, EE

Title: Comparative analysis of uncoupling protein 4 distribution in various tissues under physiological conditions and during development.

Source: Biochim Biophys Acta. 2009; 1788(10):2309-2319



Authors Vetmeduni Vienna:

Pohl Elena
Rupprecht Anne
Smorodchenko Alina


Abstract:
UCP4 is a member of the mitochondrial uncoupling protein subfamily and one of the three UCPs (UCP2, UCP4, UCP5), associated with the nervous system. Its putative functions include thermogenesis, attenuation of reactive oxidative species (ROS), regulation of mitochondrial calcium concentration and involvement in cell differentiation and apoptosis. Here we investigate UCP4"s subcellular, cellular and tissue distribution, using an antibody designed specially for this study, and discuss the findings in terms of the protein"s possible functions. Western blot and immunohistochemistry data confirmed that UCP4 is expressed predominantly in the central nervous system (CNS), as previously shown at mRNA level. No protein was found in heart, spleen, stomach, intestine, lung, thymus, muscles, adrenal gland, testis and liver. The reports revealing UCP4 mRNA in kidney and white adipose tissue were not confirmed at protein level. The amount of UCP4 varies in the mitochondria of different brain regions, with the highest protein content found in cortex. We show that UCP4 is present in fetal murine brain tissue as early as embryonic days 12-14 (E12-E14), which coincides with the beginning of neuronal differentiation. The UCP4 content in mitochondria decreases as the age of mice increases. UCP4 preferential expression in neurons and its developmental expression pattern under physiological conditions may indicate a specific protein function, e.g. in neuronal cell differentiation.

Keywords Pubmed: Age Factors
Amino Acid Sequence
Animals
Blotting, Western
Cell Differentiation
Embryo, Mammalian/cytology
Embryo, Mammalian/metabolism*
Female
Gene Expression Regulation, Developmental
Immunoenzyme Techniques
Immunoglobulin G/immunology
Ion Channels/genetics
Ion Channels/immunology
Ion Channels/metabolism*
Mice
Mice, Inbred C57BL
Mitochondria/metabolism*
Mitochondrial Proteins/genetics
Mitochondrial Proteins/immunology
Mitochondrial Proteins/metabolism*
Molecular Sequence Data
Neurons/cytology
Neurons/metabolism*
RNA, Messenger/genetics
RNA, Messenger/metabolism
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Subcellular Fractions
Tissue Distribution


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