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Publication type: Journal Article
Document type: Full Paper

Year: 2010

Author(s): Worliczek, HL; Buggelsheim, M; Alexandrowicz, R; Witter, K; Schmidt, P; Gerner, W; Saalmüller, A; Joachim, A

Title: Changes in lymphocyte populations in suckling piglets during primary infections with Isospora suis.

Source: Parasite Immunol. 2010; 32(4):232-244

Authors Vetmeduni Vienna:

Gerner Wilhelm
Joachim Anja
Saalmüller Armin
Schmidt Peter
Witter Kirsti
Worliczek Hanna

Vetmed Research Units
Institute of Immunology
Institute of Parasitology
Institute of Pathology
Institute of Topographical Anatomy

Isospora suis, a common intestinal parasite of piglets, causes neonatal porcine coccidiosis, which results in reduced and uneven weaning weights and economic losses in pig production. Nevertheless, there are no detailed studies available on the immune response to I. suis. The aim of this study was to carry out phenotypical characterization of lymphocytes during primary infections on day 3 after birth. Infected and noninfected piglets were investigated between days 7 and 16 after birth. Lymphocytes from the blood, spleen and mesenteric lymph nodes (flow cytometry) and of the jejunal mucosa (immunohistochemistry) were analysed. A decrease in T cells, especially with the phenotype of resting T-helper cells, T-cell receptor-gammadelta-T cells, and regulatory T cells in the blood, spleen and mesenteric lymph nodes was noticeable. An increase in cells with the phenotype of natural killer cells in the spleen of infected animals was found, and the subset of TcR-gammadelta-T cells was strongly increased in the gut mucosa. Our findings suggest an accelerated migration of those cells into the gut. This study provides a strong indication for the involvement of adaptive and innate immune response mechanisms in the primary immune response to I. suis, especially of TcR-gammadelta-T cells as a linkage between innate and adaptive immunity.

Keywords Pubmed: Animals
Animals, Newborn
Intestinal Mucosa/immunology
Lymph Nodes/immunology
Lymphocyte Count
Lymphocyte Subsets/immunology*
Swine Diseases/immunology*
Swine Diseases/parasitology*

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