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Type of publication: Journal Article
Type of document: Full Paper

Year: 2010

Authors: Dzieciol, M; Volgger, P; Khol, J; Baumgartner, W; Wagner, M; Hein, I

Title: A novel real-time PCR assay for specific detection and quantification of Mycobacterium avium subsp. paratuberculosis in milk with the inherent possibility of differentiation between viable and dead cells.

Source: BMC Res Notes. 2010; 3:251



Authors Vetmeduni Vienna:

Baumgartner Walter
Dzieciol Monika
Hein Ingeborg
Khol Johannes
Wagner Martin

Vetmed Research Units
University Clinic for Ruminants, Clinical Unit of Ruminant Medicine
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Food Microbiology


Abstract:
Mycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of paratuberculosis (Johne"s disease) in ruminants and is suggested to be one of the etiologic factors in Crohn"s disease in humans. Contaminated milk might expose humans to that pathogen. The aim of the present study was to develop a novel real-time PCR assay providing the additional possibility to detect viable Mycobacterium avium subsp. paratuberculosis (MAP) based on the MAP-specific Mptb52.16 target. The design included an internal amplification control to identify false negative results.


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