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Type of publication: Journal Article
Type of document: Full Paper

Year: 2010

Authors: Marek, A; Schulz, E; Hess, C; Hess, M

Title: Comparison of the fibers of Fowl adenovirus A serotype 1 isolates from chickens with gizzard erosions in Europe and apathogenic reference strains.

Source: J Vet Diagn Invest. 2010; 22(6):937-941

Authors Vetmeduni Vienna:

Hess Claudia
Hess Michael
Marek Ana

Vetmed Research Units
University Clinic for Poultry and Fish Medicine, Clinical Unit of Poultry Medicine

A total of 18 samples from 4 outbreaks of gizzard erosions in broiler chickens in Europe were used in the current study. Fowl adenoviruses were found in samples from all 4 outbreaks, and isolates were identified as Fowl adenovirus A (FAdV-A) serotype 1. As described earlier, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the long fiber gene was conducted. However, all 18 samples showed the same pattern as apathogenic FAdV-1 strains: Ote and chicken embryo lethal orphan (CELO) viruses. Nucleotide and amino acid sequences of the long and short fiber of several isolates from broiler chickens with gizzard erosions were analyzed, and 100% identity between the field isolates on the protein level was revealed. Only 1 nonsynonymous mutation (T→A) was present in the long fiber of studied isolates compared to the CELO strain. The same mutation was also present in the Ote strain. Four nonsynonymous mutations were present in the long fiber of studied isolates compared to Ote strain. In the short fiber, 6 nonsynonymous mutations were found in the studied isolates compared to the CELO strain. However, the short fiber of pathogenic isolates was 100% identical to apathogenic Ote strain. In conclusion, the usefulness of PCR-RFLP analysis of the long fiber gene of FAdV-1 isolates in distinguishing between those that induce gizzard erosions and those that do not remains questionable for the isolates obtained from European poultry flocks. The role of certain FAdV-1 strains with their long and short fiber in pathogenicity regarding gizzard erosions is still not clear.

Keywords Pubmed: Adenoviridae Infections/diagnosis
Adenoviridae Infections/epidemiology
Adenoviridae Infections/veterinary*
Adenoviridae Infections/virology
Amino Acid Sequence
Disease Outbreaks/veterinary
Fowl adenovirus A/isolation & purification*
Fowl adenovirus A/pathogenicity
Molecular Sequence Data
Poultry Diseases/epidemiology
Poultry Diseases/pathology
Poultry Diseases/virology*

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