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Type of publication: Journal Article
Type of document: Full Paper

Year: 2004

Authors: Wallner, B; Piumi, F; Brem, G; Müller, M; Achmann, R

Title: Isolation of Y chromosome-specific microsatellites in the horse and cross-species amplification in the genus Equus.

Source: J Hered. 2004; 95(2):158-164

Authors Vetmeduni Vienna:

Brem Gottfried
Müller Mathias
Wallner Barbara

Vetmed Research Units
Institute of Animal Breeding and Genetics

Y chromosome polymorphisms such as microsatellites or single nucleotide polymorphisms represent a paternal counterpart to mitochondrial DNA (mtDNA) for evolutionary and phylogeographic studies. The use of Y chromosome haplotyping in natural populations of species other than humans is still hindered by the lack of sequence information necessary for polymorphism screening. Here we used representational difference analysis (RDA) followed by a screen of a bacterial artificial chromosome (BAC) library for repetitive sequences to obtain polymorphic Y-chromosomal markers. The procedure was performed for the domestic horse (Equus caballus) and we report the first six Y-chromosomal microsatellite markers for this species. Three markers were also useful for haplotyping taxa of the zebra/ass lineage. Y-chromosomal microsatellite markers show a single haplotype in the domestic horse, whereas notable variation has been observed in the other members of the genus Equus.

Keywords Pubmed: Alleles*
Base Sequence
Chromosomes, Artificial, Bacterial
DNA Primers
Databases, Genetic
Electrophoresis, Agar Gel
Microsatellite Repeats/genetics*
Molecular Sequence Data
Polymorphism, Genetic*
Sequence Analysis, DNA
Y Chromosome/genetics*

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