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Publication type: Journal Article
Document type: Full Paper

Year: 2012

Author(s): Strasser, A; Kühnel, H; Velde, K; Dadak, A

Title: Immunomodulation during and after castration under inhalation anaesthetic without genotoxic effects on equine lymphocytes.

Source: Res Vet Sci. 2012; 92(2):306-310

Authors Vetmeduni Vienna:

Dadak Agnes
Kühnel Harald
Strasser Alois
Velde Karsten

Vetmed Research Units
University Equine Clinic, Clinical Unit of Equine Surgery
Institute of Pharmacology and Toxicology, Unit of Clinical Pharmacology
Institute of Physiology, Pathohysiology and Biophysics, Unit of Physiology, Pathophysiology, and Experimental Endocrinology

Genotoxic DNA damage due to inhalation anaesthesia has been demonstrated in human lymphocytes. In order to evaluate anaesthesia-associated changes in cell-mediated immunity on the basis of a potential DNA damage as a health risk in horses, single cell gel electrophoresis and lymphocyte proliferation assay were performed on equine lymphocytes which were obtained before, during and after regular castration under inhalation anaesthetic. No significant lymphocytic DNA damage due to isoflurane anaesthesia was observed, whereas lymphocyte proliferative reactivity and lymphocyte counts decreased significantly (p≤0.05) during and after anaesthesia. The present study thus indicates that the combined anaesthesia does not result in significant DNA damage, which hence cannot be held responsible for the observed changes in the immune response of equine lymphocytes. However, the recognized compromises of immune function ought to be considered especially in immunologically challenged animals.

Keywords Pubmed: Anesthetics, Inhalation/adverse effects
Anesthetics, Inhalation/pharmacology*
Comet Assay/veterinary
DNA Damage/drug effects*
Immunity, Cellular/drug effects*
Ketamine/adverse effects
Leukocyte Count/veterinary
Lymphocytes/drug effects*

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