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Publication type: Journal Article
Document type: Full Paper

Year: 2006

Author(s): Drillich, M; Arlt, S; Kersting, S; Bergwerff, AA; Scherpenisse, P; Heuwieser, W

Title: Ceftiofur derivatives in serum, uterine tissues, cotyledons, and lochia after fetal membrane retention.

Source: J Dairy Sci. 2006; 89(9):3431-3438



Authors Vetmeduni Vienna:

Drillich Marc


Abstract:
The objective of the study was to determine concentrations of ceftiofur derivatives after subcutaneous application of ceftiofur hydrochloride in cows with retained fetal membranes. Concentrations of ceftiofur derivatives detected as desfuroylceftiofuracetamide were determined in blood serum, endometrium, caruncles, cotyledons, and lochia during 72 h. After induction of parturition, 2 primiparous and 4 multiparous cows having retained fetal membranes for at least 12 h were studied. All cows received 3 consecutive injections (C1 to C3; 24 h apart) of 1-mg ceftiofur equivalents per kilogram of body weight as ceftiofur hydrochloride sterile suspension. Samples of blood, endometrium, caruncles, cotyledons, and lochia were collected immediately before each injection (0 h) and again at 4, 12, and 24 h after C1, C2, and C3. Blood samples were collected from coccygeal vessels. Caruncles were removed from the uterine lumen by manual extirpation and separated from cotyledons. Endometrial tissue (0.5 g) was collected by using Kenny"s biopsy apparatus. For all samples, concentrations of potentially active ceftiofur derivatives were quantified using an HPLC assay. Within 2 h (serum), 4 h (endometrium), and 12 h (caruncles, cotyledons, lochia) after C1 and during the entire study period, mean concentration of ceftiofur derivatives exceeded the reported minimum drug concentrations required to inhibit the growth of 90% of isolates for relevant bacteria such as Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes. Only in single samples did concentrations decrease temporarily below the reported minimum drug concentrations required to inhibit the growth of 90% of isolates.

Keywords Pubmed: Animals
Anti-Bacterial Agents/administration & dosage
Anti-Bacterial Agents/blood
Anti-Bacterial Agents/pharmacokinetics*
Body Temperature
Cattle/metabolism*
Cattle Diseases/drug therapy
Cattle Diseases/metabolism
Cephalosporins/administration & dosage
Cephalosporins/blood
Cephalosporins/pharmacokinetics*
Endometrium/chemistry
Endometrium/metabolism*
Extraembryonic Membranes/chemistry
Extraembryonic Membranes/metabolism*
Female
Injections, Subcutaneous/veterinary
Placenta/chemistry
Placenta/metabolism
Placenta, Retained/drug therapy
Placenta, Retained/metabolism
Placenta, Retained/veterinary*
Pregnancy
Serum/chemistry
Time Factors


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