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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2011

Author(s): Käser, T; Gerner, W; Saalmüller, A

Title: Porcine regulatory T cells: mechanisms and T-cell targets of suppression.

Source: Dev Comp Immunol. 2011; 35(11):1166-1172



Authors Vetmeduni Vienna:

Gerner Wilhelm
Käser Tobias
Saalmüller Armin

Vetmed Research Units
Institute of Immunology


Abstract:
Tregs are known for their suppressive capacity on various immune reactions. In swine, existence as well as suppressive activity of Foxp3(+) Tregs could be demonstrated but detailed functional investigations are lacking. Therefore, we analysed the functional properties of porcine Tregs. We observed that besides TCR stimulation Tregs require IL-2 for activation. Furthermore, we investigated the following mechanisms of suppression: (i) cell-cell contact dependency, (ii) production of soluble suppressive factors and (iii) competition for growth factors. Our experiments revealed that suppression by porcine Tregs is abrogated by blocking cell-cell contact or by supplementing excessive amounts of IL-2. Additionally it could be shown that porcine Tregs produce immunosuppressive IL-10. Thereby, we demonstrated that porcine Tregs can use all main mechanisms of suppression mentioned above. Further investigations on the suppressive activity of Tregs using CFSE proliferation assays demonstrated that suppression affects T-helper cells as well as cytotoxic T lymphocytes and TCR-γδ T cells.

Keywords Pubmed: Animals
CD4-Positive T-Lymphocytes/immunology
Cell Communication
Forkhead Transcription Factors/biosynthesis
Forkhead Transcription Factors/metabolism
Immune Tolerance*
Interleukin-10/biosynthesis
Interleukin-10/immunology
Interleukin-2*/biosynthesis
Interleukin-2*/immunology
Interleukin-2*/metabolism
Lymphocyte Activation*
Receptors, Antigen, T-Cell, gamma-delta/immunology
Receptors, Antigen, T-Cell, gamma-delta/metabolism
Swine/immunology*
T-Lymphocytes, Cytotoxic/immunology
T-Lymphocytes, Helper-Inducer/immunology
T-Lymphocytes, Helper-Inducer/metabolism
T-Lymphocytes, Regulatory/cytology
T-Lymphocytes, Regulatory/immunology*
T-Lymphocytes, Regulatory/metabolism


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