Effect of reproductive tract environment following controlled ovarian hyperstimulation treatment on embryo development and global transcriptome profile of blastocysts: implications for animal breeding and human assisted reproduction.
In mammals, the reproductive tract plays a crucial role in the success of early reproductive events and provides an optimal microenvironment for early embryonic development. However, changes in the reproductive tract environment associated with controlled ovarian hyperstimulation and the influence on the embryo transcriptome profile have not been investigated. Therefore, we investigated differences in the development rate and the transcriptome profile of bovine blastocysts developing in the reproductive tract of unstimulated or superovulated heifers.
Animals Blastocyst/metabolism Blastocyst/physiology* Breeding Cattle Cluster Analysis Electron Transport/genetics Electron Transport Complex III/genetics Electron Transport Complex III/metabolism Embryo Transfer Embryonic Development/drug effects* Embryonic Development/genetics Embryonic Development/physiology Female Gene Expression Profiling Humans Insemination, Artificial Oviducts/drug effects* Oviducts/metabolism Ovulation Induction* Oxidative Phosphorylation Pregnancy Superovulation Uterus/drug effects* Uterus/metabolism