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Publication type: Journal Article
Document type: Full Paper

Year: 2011

Author(s): Metzler-Zebeli, BU; Zijlstra, RT; Mosenthin, R; Gänzle, MG

Title: Dietary calcium phosphate content and oat β-glucan influence gastrointestinal microbiota, butyrate-producing bacteria and butyrate fermentation in weaned pigs.

Source: FEMS Microbiol Ecol. 2011; 75(3):402-413



Authors Vetmeduni Vienna:

Metzler-Zebeli Barbara


Abstract:
This study aimed to evaluate the effects of oat β-glucan in combination with low- and high-dietary calcium phosphate (CaP) content on gastrointestinal bacterial microbiota, prevalence of butyrate-production pathway genes and fermentation end-products in 32 weaned pigs allocated to four diets: a cornstarch-casein-based diet with low [65% of the calcium (Ca) and phosphorous (P) requirement] and high CaP content (125% and 115% of the Ca and P requirement, respectively); and low and high CaP diets supplemented with 8.95% of oat β-glucan concentrate. Pigs were slaughtered after 14 days, and digesta were collected for quantitative PCR analysis, and quantification of short-chain fatty acids and lactate. The high CaP content reduced gastric lactate and streptococci and propionate in the large intestine. Oat β-glucan distinctly raised gastric bacterial numbers, and colonic lactobacilli and bifidobacteria. Although not reflected by gene copies of butyrate-production pathway genes, oat β-glucan also increased gastric, caecal and colonic butyrate concentrations, which may be favourable for intestinal development in weaned pigs. Thus, a high CaP content negatively affected the intestinal abundance of certain fermentation end-products, whereas oat β-glucan generally enhanced bacterial numbers and activity. The results emphasize the importance of the stomach for bacterial metabolism of oat β-glucan in weaned pigs.

Keywords Pubmed: Animals
Avena sativa/metabolism
Bacteria/classification
Bacteria/genetics
Bacteria/metabolism*
Butyrates/metabolism*
Calcium Phosphates/metabolism*
Calcium, Dietary/metabolism*
Diet/veterinary
Fatty Acids, Volatile/analysis
Fermentation*
Gastrointestinal Contents/chemistry
Gastrointestinal Contents/microbiology
Gastrointestinal Tract/microbiology*
Gene Dosage
Lactic Acid/analysis
Multivariate Analysis
RNA, Ribosomal, 16S/genetics
Random Allocation
Swine
Weaning
beta-Glucans/metabolism*


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