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Type of publication: Journal Article
Type of document: Full Paper

Year: 2012

Authors: Held, E; Mertens, EM; Mohammadi-Sangcheshmeh, A; Salilew-Wondim, D; Besenfelder, U; Havlicek, V; Herrler, A; Tesfaye, D; Schellander, K; Hölker, M

Title: Zona pellucida birefringence correlates with developmental capacity of bovine oocytes classified by maturational environment, COC morphology and G6PDH activity.

Source: Reprod Fertil Dev. 2012; 24(4):568-579



Authors Vetmeduni Vienna:

Besenfelder Urban
Havlicek Vitezslav

Vetmed Research Units
Institute of Animal Breeding and Genetics, Unit of Reproductive Biology


Abstract:
In the present study we aimed to analyse structural changes during in vitro maturation of the bovine zona pellucida (ZP) by scanning electron microscopy (SEM) ands zona pellucida birefringence (ZPB). Here we show that alterations during in vitro maturation invasively analysed by SEM are reflected in ZPB. In vivo-matured oocytes displayed significantly lower birefringence parameters and significantly higher blastocyst rates compared with in vitro-derived oocytes (39.1% vs 21.6%). The same was observed for in vitro-matured oocytes with cumulus-oocyte complex (COC) Quality 1 (Q1) compared with Q3-COCs with respect to zona birefringence and developmental capacity. Immature oocytes with Q1-COCs displayed higher ZPB values and a higher developmental capacity to the blastocyst stage (27.7% vs 16.9%) compared with immature Q3-COCs. Considering in vitro-matured oocytes, only those with Q1-COC showed a trend for ZPB similar to in vivo-matured oocytes. Therefore, a decreasing trend for ZPB during in vitro maturation seems to be typical for high-quality oocytes and successful cytoplasmic maturation. In accordance, fully-grown immature oocytes reached significantly higher blastocyst rates (32.0% vs 11.5%) and lower ZPB values compared with still-growing ones. In conclusion, we successfully evaluated the applicability of zona imaging to bovine oocytes: alterations during in vitro maturation invasively analysed by scanning electron microscopy were reflected in the birefringence of the zona pellucida of bovine oocytes affecting developmental capacity at the same value. Therefore ZPB measurement by live zona imaging has potential to become a new tool to assess correctness of in vitro maturation and to predict developmental competence.

Keywords Pubmed: Animals
Birefringence
Blastocyst/enzymology
Blastocyst/physiology
Blastocyst/ultrastructure
Cattle/physiology*
Cell Survival
Coloring Agents/metabolism
Cumulus Cells/enzymology
Cumulus Cells/physiology*
Cumulus Cells/ultrastructure
Female
Fertilization in Vitro/veterinary
Glucosephosphate Dehydrogenase/metabolism*
Microscopy, Electron, Scanning/veterinary
Oocytes/enzymology
Oocytes/physiology*
Oocytes/ultrastructure
Oogenesis*
Oxazines/metabolism
Single-Cell Analysis/methods
Single-Cell Analysis/veterinary*
Zona Pellucida/chemistry*
Zona Pellucida/physiology
Zona Pellucida/ultrastructure


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