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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2012

Author(s): Reutner, K; Leitner, J; Essler, SE; Witter, K; Patzl, M; Steinberger, P; Saalmüller, A; Gerner, W

Title: Porcine CD27: identification, expression and functional aspects in lymphocyte subsets in swine.

Source: Dev Comp Immunol. 2012; 38(2):321-331



Authors Vetmeduni Vienna:

Gerner Wilhelm
Hammer Sabina
Patzl Martina
Reutner Katharina
Saalmüller Armin
Witter Kirsti

Vetmed Research Units
Institute of Immunology
Institute of Topographical Anatomy


Abstract:
Up to now for Swine Workshop Cluster 2 (SWC2) the orthologous human CD molecule was unknown. By use of the SWC2-specific mAb b30c7 and a retroviral cDNA expression library derived from stimulated porcine peripheral blood mononuclear cells we could identify SWC2 as porcine CD27. Phenotypic analyses of lymphocytes isolated from blood and lymphatic organs revealed that mature T cells in thymus and T cells in the periphery with a naïve phenotype were CD27(+). However, within CD8α(+) T helper and CD8α(+) γδ T cells also CD27(-) cells were present, indicating a down-regulation after antigen contact in vivo. B cells lacked CD27 expression, whereas NK cells expressed intermediate levels. Furthermore, plate-bound mAb b30c7 showed a costimulatory capacity on CD3-activated T cells for proliferation, IFN-γ and TNF-α production. Hence, our data indicate an important role of porcine CD27 for T-cell differentiation and activation as described for humans and mice.

Keywords Pubmed: Amino Acid Sequence
Animals
Antigens, CD27/chemistry
Antigens, CD27/genetics
Antigens, CD27/immunology*
B-Lymphocytes/immunology
Cell Differentiation*
Humans
Killer Cells, Natural/immunology
Lymphocyte Activation*
Molecular Sequence Data
Sequence Alignment
T-Lymphocyte Subsets/immunology
T-Lymphocytes/cytology*
T-Lymphocytes/immunology
T-Lymphocytes/metabolism


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